Label the individual strips that will be used and aliquot reagents as the following example:
Component | Volume per Reaction | 24 Reactions |
Kanamycin Antibody #1 | 50uL | 1.2mL |
HRP-Conjugated Ab #2 | 50uL | 1.2mL |
1X Wash Solution | 1.0 mL | 24mL |
Stop Buffer | 100 uL | 2.4 mL |
TMB Substrate | 100 uL | 2.4 mL |
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