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| Place of Origin | USA |
| Brand Name | REAGEN |
| Certification | FAPAS |
| Model Number | RND99005 |
Nitroimidazole ELISA Test Kit
REAGEN™ Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.
1. High recovery (70% - 95%), and cost-effective extraction methods for various samples.
2. A quick ELISA assay (less than 1.5 hours regardless of number of samples).
3. High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The Nitroimidazole antibody has been coated in the plate wells. During the analysis, sample is added along with the Nitroimidazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the Nitroimidazole antibody, thereby preventing the Nitroimidazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat/Liver/Kidney/Fish/ Honey | 0.25 |
| Shrimp | 0.25 |
| Analytes | Cross-Reactivity (%) |
| Dimetridazole | 100 |
| Metronidazole | 93.4 |
| Hydroxydimetridazole | 51.3 |
| Hydroxymetronidazole | ＞100 |
| Ronidazole | ＞100 |
| Ipronidazole | ＞100 |
| Hydroxyipronidazole | 66.1 |
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