Domoic Acid (ASP) ELISA Test Kit
REAGEN™Domoic Acid (ASP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Domoic Acid in mussels, algae and water samples.
1. High recovery (>80%), rapid (10 - 30 minutes), and cost-effective extraction methods
2. High sensitivity (0.125ppb) and low detection limit (30ppb for mussel samples and 6ppb for water samples)
3. High reproducibility.
4. A quick ELISA assay(less than 1.5 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Domoic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the Domoic Acid-horseradish peroxidase conjugate (Domoic Acid-HRP Conjugate) and Anti- Domoic Acid Antibody. If the Domoic Acid residue is present in the sample, it will compete with Domoic Acid-HRP Conjugate for the Anti-Domoic Acid Antibody, thereby preventing the Domoic Acid-HRP Conjugate from binding to the Anti-Domoic Acid Antibody. The Anti-Domoic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the domoic acid residue concentration in the sample.
|Sample Type||Detection Limit (ng/g or ppb)|
|Domoic Acid(including isomers)||100|
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