High Sensitive L-Carnitine Colorimetric Assay Kit Milk Powder Testing Kit

L-Carnitine Colorimetric Assay Kit

product Description

REAGEN^™ L-Carnitine Test Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as milk powder.

the unique features of the kit are:

1.Low detection limit(2mg/100g)

2.High reproducibility

3.A rapid robust enzyme-based assay.

procedure Overview

Carnitine is a quaternary ammonium compound biosynthesized from lysine and methionine. It is required for fatty acid transport into the mitochondrial matrix via the carnitine/ acylcarnitine shuttle, where β-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.

REAGEN^™ L-Carnitine Test Kit assay transfers an acetyl group from CoA to Carnitine and the free CoA formed is further processed with substrate to give absorbance (405 nm).

Kit Contents, Storage and Shelf Life

REAGEN^™ L-Carnitine Test Kit Store the kit at 4°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.