REAGEN™ L-Carnitine Test Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as milk powder.
1.Low detection limit(2mg/100g)
3.A rapid robust enzyme-based assay.
Carnitine is a quaternary ammonium compound biosynthesized from lysine and methionine. It is required for fatty acid transport into the mitochondrial matrix via the carnitine/ acylcarnitine shuttle, where β-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.
REAGEN™ L-Carnitine Test Kit assay transfers an acetyl group from CoA to Carnitine and the free CoA formed is further processed with substrate to give absorbance (405 nm).
|5 x Carnitine Substrate||3ml/bottle,1bottle|
|L-Carnitine Standard||32ug ,3bottle|
REAGEN™ L-Carnitine Test Kit Store the kit at 4°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
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