Food Safety Enrofloxacin ELISA Test Kit Rapid Assay Protocol With Strong Sensitivity

Basic Information
Place of Origin: USA
Brand Name: REAGEN
Certification: FAPAS
Model Number: RND99041
Minimum Order Quantity: 5 kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-7 days
Payment Terms: T/T
Supply Ability: 100 kits per month
Detail Information
Specimen: Meat/Liver/Kidney/Fish/Shrimp Size: 96 Test
Package: Color Packing Shelf Life: One Year
Keywords: Enrofloxacin Test Kit , Elisa Kit , Enrofloxacin Elisa Test Kit Sensitivity: 0.1 Ng/ml
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Product Description

Enrofloxacin ELISA Test Kit

Product Description

REAGEN Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doxycycline in egg, honey, meat, fish , shrimp and milk.


The unique features of the kit are:

  • High recovery (75-120%), rapid and cost-effective extraction methods.

  • High sensitivity (0.1ng/g or ppb) and low detection limit for samples.

  • High reproducibility.

  • A quick ELISA assay (less than 1 hours regardless of number of samples).


Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The doxycycline-BSA has been coated in the plate wells. During the analysis, sample and the doxycycline antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the doxycycline residue is present in the sample, it will compete for the doxycycline antibody, thereby preventing the antibody from binding to the doxycycline-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the doxycycline residue concentration in the sample.


Food Safety Enrofloxacin ELISA Test Kit Rapid Assay Protocol With Strong Sensitivity 0


Kit Contents, Storage and Shelf Life

REAGEN Doxycycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.


Kit Contents Amount Storage
Doxycycline-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃

Doxycycline Standards:

Negative control (white Cap tube)

0.1ng/mL (yellow Cap tube)

0.3 ng/mL (orange Cap tube)

0.9 ng/mL (pink Cap tube)

2.7 ng/mL (purple Cap tube)

8.1 ng/mL (blue Cap tube)

500ng/mL (Spiking, red Cap tube)


1.0 mL

1.0 mL

1.0 mL


1.0 mL

1.0 mL

1.0 mL







Doxycycline Antibody #1 6 mL 2-8℃
HRP-Conjugated Ab #2 6 mL
20X Wash Solution 30 mL
10X Sample Ext. Buffer 30 mL
Stop Buffer 12 mL
TMB Substrate 12 mL

If you are not planning to use the kit for over 1 month, storing Doxycycline Standard Stock, Doxycycline Antibody #1 and HRP-Conjugated Ab #2 at -20℃ or in a freezer is recommended.



Sample Type Detection Limit (ng/g or ppb)
Egg 1.5
Honey/Milk 1
Meat/Fish/Shrimp 1



Analytes Cross-Reactivity (%)
Doxycycline 100
Chlortetracycline 24
Oxytetracycline 21
Tetracycline 15


Contact Details

Phone Number : 856-727-0250

WhatsApp : +8615361523839