drug residue testing kits,
drug residue elisa kit
REAGEN™ Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doxycycline in egg, honey, meat, fish , shrimp and milk.
1.High recovery (75-120%), rapid and cost-effective extraction methods.
2.High sensitivity (0.1ng/g or ppb) and low detection limit for samples.
4.A quick ELISA assay (less than 1 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The doxycycline-BSA has been coated in the plate wells. During the analysis, sample and the doxycycline antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the doxycycline residue is present in the sample, it will compete for the doxycycline antibody, thereby preventing the antibody from binding to the doxycycline-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the doxycycline residue concentration in the sample.
REAGEN™ Doxycycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.
|Doxycycline-Coated Plate||1 x 96-well plate (8 wells x 12 strips)||2-8℃|
Negative control (white Cap tube)
0.1ng/mL (yellow Cap tube)
0.3 ng/mL (orange Cap tube)
0.9 ng/mL (pink Cap tube)
2.7 ng/mL (purple Cap tube)
8.1 ng/mL (blue Cap tube)
500ng/mL (Spiking, red Cap tube)
|Doxycycline Antibody #1||6 mL||2-8℃|
|HRP-Conjugated Ab #2||6 mL|
|20X Wash Solution||30 mL|
|10X Sample Ext. Buffer||30 mL|
|Stop Buffer||12 mL|
|TMB Substrate||12 mL|
If you are not planning to use the kit for over 1 month, storing Doxycycline Standard Stock, Doxycycline Antibody #1 and HRP-Conjugated Ab #2 at －20℃ or in a freezer is recommended.
|Sample Type||Detection Limit (ng/g or ppb)|