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Colorimetric Doxycycline ELISA Test Kit Quantitative Analysis High Recovery

Colorimetric Doxycycline ELISA Test Kit Quantitative Analysis High Recovery

MOQ: 5 kits
Price: Negotiable
Standard Packaging: color packing
Delivery Period: 5-7 days
Payment Method: T/T
Supply Capacity: 100 kits per month
Detail Information
Place of Origin
USA
Brand Name
REAGEN
Model Number
RND99048
Highlight:

drug residue testing kits

,

drug residue elisa kit

Product Description

Doxycycline ELISA Test Kit

Product Description

REAGEN Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doxycycline in egg, honey, meat, fish , shrimp and milk.

 

The unique features of the kit are:

1.High recovery (75-120%), rapid and cost-effective extraction methods.

2.High sensitivity (0.1ng/g or ppb) and low detection limit for samples.

3.High reproducibility.

4.A quick ELISA assay (less than 1 hours regardless of number of samples).

 

Colorimetric Doxycycline ELISA Test Kit Quantitative Analysis High Recovery 0

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The doxycycline-BSA has been coated in the plate wells. During the analysis, sample and the doxycycline antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the doxycycline residue is present in the sample, it will compete for the doxycycline antibody, thereby preventing the antibody from binding to the doxycycline-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the doxycycline residue concentration in the sample.

 

Kit Contents, Storage and Shelf Life

REAGEN Doxycycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

 

Kit Contents Amount Storage
Doxycycline-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃

Doxycycline Standards:

Negative control (white Cap tube)

0.1ng/mL (yellow Cap tube)

0.3 ng/mL (orange Cap tube)

0.9 ng/mL (pink Cap tube)

2.7 ng/mL (purple Cap tube)

8.1 ng/mL (blue Cap tube)

500ng/mL (Spiking, red Cap tube)

 

1.0 mL

1.0 mL

1.0 mL

1.0mL

1.0 mL

1.0 mL

1.0 mL

 

 

2-8℃

 

 

 

Doxycycline Antibody #1 6 mL 2-8℃
HRP-Conjugated Ab #2 6 mL
20X Wash Solution 30 mL
10X Sample Ext. Buffer 30 mL
Stop Buffer 12 mL
TMB Substrate 12 mL
 

If you are not planning to use the kit for over 1 month, storing Doxycycline Standard Stock, Doxycycline Antibody #1 and HRP-Conjugated Ab #2 at -20℃ or in a freezer is recommended.

 

Sensitivity

Sample Type Detection Limit (ng/g or ppb)
Egg 1.5
Honey/Milk 1
Meat/Fish/Shrimp 1
 

Specificity

Analytes Cross-Reactivity (%)
Doxycycline 100
Chlortetracycline 24
Oxytetracycline 21
Tetracycline 15
 
products
PRODUCTS DETAILS
Colorimetric Doxycycline ELISA Test Kit Quantitative Analysis High Recovery
MOQ: 5 kits
Price: Negotiable
Standard Packaging: color packing
Delivery Period: 5-7 days
Payment Method: T/T
Supply Capacity: 100 kits per month
Detail Information
Place of Origin
USA
Brand Name
REAGEN
Model Number
RND99048
Minimum Order Quantity:
5 kits
Price:
Negotiable
Packaging Details:
color packing
Delivery Time:
5-7 days
Payment Terms:
T/T
Supply Ability:
100 kits per month
Highlight

drug residue testing kits

,

drug residue elisa kit

Product Description

Doxycycline ELISA Test Kit

Product Description

REAGEN Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doxycycline in egg, honey, meat, fish , shrimp and milk.

 

The unique features of the kit are:

1.High recovery (75-120%), rapid and cost-effective extraction methods.

2.High sensitivity (0.1ng/g or ppb) and low detection limit for samples.

3.High reproducibility.

4.A quick ELISA assay (less than 1 hours regardless of number of samples).

 

Colorimetric Doxycycline ELISA Test Kit Quantitative Analysis High Recovery 0

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The doxycycline-BSA has been coated in the plate wells. During the analysis, sample and the doxycycline antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the doxycycline residue is present in the sample, it will compete for the doxycycline antibody, thereby preventing the antibody from binding to the doxycycline-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the doxycycline residue concentration in the sample.

 

Kit Contents, Storage and Shelf Life

REAGEN Doxycycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

 

Kit Contents Amount Storage
Doxycycline-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃

Doxycycline Standards:

Negative control (white Cap tube)

0.1ng/mL (yellow Cap tube)

0.3 ng/mL (orange Cap tube)

0.9 ng/mL (pink Cap tube)

2.7 ng/mL (purple Cap tube)

8.1 ng/mL (blue Cap tube)

500ng/mL (Spiking, red Cap tube)

 

1.0 mL

1.0 mL

1.0 mL

1.0mL

1.0 mL

1.0 mL

1.0 mL

 

 

2-8℃

 

 

 

Doxycycline Antibody #1 6 mL 2-8℃
HRP-Conjugated Ab #2 6 mL
20X Wash Solution 30 mL
10X Sample Ext. Buffer 30 mL
Stop Buffer 12 mL
TMB Substrate 12 mL
 

If you are not planning to use the kit for over 1 month, storing Doxycycline Standard Stock, Doxycycline Antibody #1 and HRP-Conjugated Ab #2 at -20℃ or in a freezer is recommended.

 

Sensitivity

Sample Type Detection Limit (ng/g or ppb)
Egg 1.5
Honey/Milk 1
Meat/Fish/Shrimp 1
 

Specificity

Analytes Cross-Reactivity (%)
Doxycycline 100
Chlortetracycline 24
Oxytetracycline 21
Tetracycline 15
 
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