β-lactamase ELISA Kit , competitive enzyme immunoassay , detect beta-lactam containing antibiotics

Basic Information
Place of Origin: USA
Brand Name: REAGEN
Certification: FAPAS
Model Number: RND99082
Minimum Order Quantity: 5 kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-7 days
Payment Terms: T/T
Supply Ability: 100 kits per month
Detail Information
Type: Reagent Size: 96 Test
Package: Color Packing Shelf Life: One Year
Sensitivity: 1ppb
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Product Description

β-lactamase ELISA Kit


This kit allows for the determination of β-lactamase activity in milk.


Principle of the assay

The kit assay β-lactamase level in the sample,use Purified β-lactamase antibody to coat microtiter plate wells, make solid-phase antibody, then add β-lactamase to wells, Combined β-lactamase antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of β-lactamase in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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Materials provided with the kit


Reqired Materials Not Provided With the Kit

  • Microtiter plate reader (450 nm)
  • Incubator
  • Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
  • Vortex mixer (e.g. Gneie Vortex mixer from VWR)
  • 10, 20, 100 and 1000 mL pipettes
  • Multi-channel pipette: 50-300 mL (Optional)
  • n-Hexane
  • Distilled deionized water
  • Disposable plastic reservoirs
  • Paper towels
  • Centrifuge

Contact Details

Phone Number : 856-727-0250

WhatsApp : +8615361523839