Fructose / Glucose Assay Kit Food Composition Testing Robust Enzyme Based Assay

Basic Information
Place of Origin: USA
Brand Name: REAGEN
Certification: FAPAS
Model Number: RNC95009
Minimum Order Quantity: 5 kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-7 days
Payment Terms: T/T
Supply Ability: 100 kits per month
Detail Information
Type: Reagent Size: 96 Test
Package: Color Packing Shelf Life: One Year
Sensitivity: 0.8ppb
High Light:

food source testing kit


food ingredients testing

Product Description

Fructose/Glucose Assay Kit

Product Description

REAGEN Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples.


The unique features of the kit are:

1.High sensitivity and low detection limit (0.8 ppm)

2.High reproducibility

3.A rapid (30 minute), robust enzyme-based assay


Procedure Overview

REAGEN Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.


Kit Contents, Storage and Shelf Life

REAGEN Fructose/Glucose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.


Kit Contents Amount Storage
Dilution Buffer 10 mL 4ºC
Substrate Mix 20 mL 4ºC
Hexokinase 0.7 mL 4ºC
PGI Enzyme 0.2 mL 4ºC
Microtiter Plate One 4ºC

Sensitivity (Detection Limit)

Sample Volume Detection Limit (mg/L)
0.01 mL 8
0.1mL 0.8


                               Fructose / Glucose Assay Kit Food Composition Testing Robust Enzyme Based Assay 0

Contact Details

Phone Number : 856-727-0250

WhatsApp : +8615361523839