Fructose/Glucose Assay Kit
1.High sensitivity and low detection limit (0.8 ppm)
3.A rapid (30 minute), robust enzyme-based assay
REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).
The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.
REAGEN™ Fructose/Glucose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
|Dilution Buffer||10 mL||4ºC|
|Substrate Mix||20 mL||4ºC|
|PGI Enzyme||0.2 mL||4ºC|
|Sample Volume||Detection Limit (mg/L)|
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