7 Minutes Serum ELISA Vitamin D3 Strip Test Kit

7 Minutes Serum ELISA Vitamin D3 Strip Test Kit

Product Description

REAGEN™Vitamin D3 Strip Test is a qualitative and rapid lateral flow assay designed to detect Vitamin D3 residues in serum. This test uses a Vitamin D3 receptor binding protein which eliminates the need for a heating block. This test is designed for point-of-care use or reference lab settings.

The unique features of the kit are:

 Rapid strip test method – 7 minutes

 Novel binding protein that requires no heating step

 High sensitivity

 High reproducibility

Mechanism of Reaction

REAGEN™ The assay uses a competitive colloidal gold based format. 200uL of the serum sample to be tested is added to a transparent test paper card plastic reaction well or tube, which is used to make the lyophilized reagent a uniform pink suspension at the bottom of the microwell. A brief 3 minute incubation allowed the gold particle-labeled Vitamin D3 complexing protein to bind to Vitamin D3 in the serum. Then insert the test strip vertically into the sample hole in the direction of the arrow, so that the sample flows to the test strip. All gold-labeled particles that are not bound to vitamin D3 in the serum will bind to Vitamin D3 on the test line (T-line). A red marker line is formed at the detection

line position.

Kit Contents, Storage and Shelf Life

The REAGEN™ Vitamin D3 Strip Test has the capacity for 96(RNS92075-01) or 192 (RNS92075-02)determinations. The shelf life is 12 months when the kit is properly stored.

A 96-well plastic frame is also provided in each kit to anchor the reaction vessels in place during

testing, and can be re-used as required