0.5ppb Rapid Tetracycline ELISA Test Kit For Fish / Shrimp

Tetracycline(TET) ELISA Test Kit 

Product Description

REAGEN^™ Tetracycline (TET) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Tetracycline in in honey, butter, whey, egg, cheese, milk, meat, meat products such as sausage, fish and shrimp.

Detection Limit Of Quantitation

Specificity (Cross-Reactivity)

The unique features of the kit are:

1. High recovery (75-120%), rapid (less than 30 minutes), and cost-effective extraction methods.

2. High sensitivity (0.1 ng/g or ppb) and low detection limit for samples.

3. High reproducibility.

4. A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

REAGEN^™ Tetracycline (TET) ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Reagent Preparation

IMPORTANT: All reagents should be brought up to room temperature before use (1 – 2 hours at 20 – 25°C / 68 – 77°F); Make sure you read “Warnings and Precautions” section. Solutions should be prepared just prior to ELISA test. All reagents should be mixed by gently inverting or swirling prior to use. Prepare volumes that are needed for the number of wells being run. Do not return the reagents to the original stock tubes/bottles. Using disposable reservoirs when handling reagents can minimize the risk of contamination and is recommended.

Preparation of 1X Wash Solution

Mix 1 volume of the 20X Wash Solution with 19 volumes of distilled water.

Preparation of 1X Standards

Mix 20 mL of the Standards with 180 mL of 1X Sample Dilution.

Special Notes for Optimal ELISA Performance

1) Allow the entire kit to equilibrate at room temperature for at least two hours before starting any ELISA assay.

2) Avoid light as much as possible during sample preparation and ELISA assay.

3) For plate washing steps: after addition of 250μL wash buffer to the wells, incubate the plate for 20 – 30 seconds; shake the plate gently before pouring out the wash buffer. Repeat this procedure for each of the 4 washes.

4) Pipette all reagents and samples very accurately, especially the samples, even if you must slow down while pipetting.