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Drug Testing Gentamicin ELISA Test Kit High Recovery Reagent Type 0.02ppb Sensitivity

Basic Information
Place of Origin: USA
Brand Name: REAGEN
Certification: FAPAS
Model Number: RND99004
Minimum Order Quantity: 5 kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-7 days
Payment Terms: T/T
Supply Ability: 100 kits per month
Detail Information
Type: Reagent Size: 96 Test
Package: Color Packing Shelf Life: One Year
Sensitivity: 0.02ppb Use: Meat , Fish , Shrimp , Milk , Urine Or Blood
High Light:

Gentamicin ELISA Test Kit

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0.02ppb Gentamicin ELISA Test Kit

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High Recovery Gentamicin ELISA Kit


Product Description

Gentamicin ELISA Test Kit

Product Description

REAGEN Gentamicin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Gentamicin in egg, honey, feed, tissue( liver/meat/chicken/fish), urine,serum,milk and milk powder.

 

The unique features of the kit are:

1. High recovery in a variety of samples, rapid , and cost-effective extraction methods.

2. High sensitivity (0.02 ng/g or ppb)

3. High reproducibility.

4. A quick ELISA assay (less than 1 hours regardless of number of samples).

 

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

 

Kit Contents, Storage and Shelf Life

REAGENGentamicin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

 

Kit Contents Amount Storage
Gentamicin-coated Plate 1x96-well Plate(8wells x 12 strips) 2-8°C

Gentamicin Standards:

Negative control (white cap tube)

0.02 ng/mL (yellow cap tube)

0.06ng/mL (orange cap tube)

0.2 ng/mL (pink cap tube)

0.6ng/mL (purple cap tube)

1.5ng/mL (blue cap tube)

50 ng/mL (spiking, optional, red cap tube)

 

1.0 mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

2-8°C
Gentamicin Antibody #1 6 mL 2-8°C
HRP-Conjugated Antibody #2 6.0 mL 2-8°C
10X Sample Extraction Buffer 30 mL 2-8°C
10X Sample Dilution Buffer 30 mL 2-8°C
20X Wash Solution 30 mL 2-8°C
Stop Buffer 12 mL 2-8°C
TMB Substrate 12 mL 2-8°C
10X Gentamicin Extraction Buffer (Optional) 30 mL 2-8°C
Gentamicin Balance Buffer (Optional) 3.0 mL 2-8°C
 

* If you are not planning to use the kit for over 1 months, store Gentamicin Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

 

Sensitivity (Detection Limit)

 

Sample Type Detection Limit (ng/g or ppb)
Egg 0.2
Feed 0.5
Honey 0.2
Tissue(liver/meat/chicken/fish) 0.2
Milk 0.02
Milk Powder 0.2
Urine/Serum 0.4
 

 

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Gentamicin 100

Contact Details
Nicole

Phone Number : 856-727-0250

WhatsApp : +8615361523839