MOQ: | 1 kits |
Price: | Negotiable |
Standard Packaging: | color packing |
Delivery Period: | 1 working day |
Payment Method: | T/T |
Supply Capacity: | 100 kits per month |
REAGEN™ BTS Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect beta-lactam, tetracycline, and sulfonamide antibiotic residues in raw commingled cow’s milk. This test uses a uses novel antibiotic binding proteins which are highly active in cold milk and eliminate the need for a heating block. This test is designed for rapid field use or reference lab settings.
1 ·Rapid strip test method –9 minutes
2 ·Works in cold milk
3 ·Novel binding proteins that requires no heating step
4 ·High sensitivity
Kit Contents |
Amount |
Storage |
Reaction wells |
12x8 (96 total) |
RT(25˚C+/- 2.5) (Dry and Dark) |
Test strips |
48x2 (96 total) |
|
Positive control |
1 vial |
|
Negative control |
1 vial |
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink or light red color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the antibiotic binding proteins on the gold particles to engage with any antibiotic present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to their respective test line, forming a signal (red line) at that position. Each test strip is imprinted with three test lines and one Control line (C-line)at the top. The top test line specifically detects for beta-lactams, the middle test line detects for tetracyclines, and the bottom test line detects for sulfonamide antibiotics. If the binding protein on the gold particle has engaged with either beta-lactams,tetracyclines, or sulfonamide antibiotic residues present in the milk sample, the gold particle will flow past the test line corresponding to that antibiotic and reach the C-line.
Negative: Test line and the C-line both appear ,indicates the milk sample is negative .
Positive: Test line which corresponding position is no colored and the C-line appear, indicates the milk sample is positive for the corresponding drug .
The test is invalid if no colored line appears in the C-line area even if a colored line appears in the test line area. Re-test milk sample if broken lines are observed. Colored lines which appear after 20 minutes are not diagnostic and should be ignored. If the sample does not flow up the dipstick, the milk sample may be clotted, invalidating the test result. To avoid milk sample clotting, always use fresh milk.
MOQ: | 1 kits |
Price: | Negotiable |
Standard Packaging: | color packing |
Delivery Period: | 1 working day |
Payment Method: | T/T |
Supply Capacity: | 100 kits per month |
REAGEN™ BTS Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect beta-lactam, tetracycline, and sulfonamide antibiotic residues in raw commingled cow’s milk. This test uses a uses novel antibiotic binding proteins which are highly active in cold milk and eliminate the need for a heating block. This test is designed for rapid field use or reference lab settings.
1 ·Rapid strip test method –9 minutes
2 ·Works in cold milk
3 ·Novel binding proteins that requires no heating step
4 ·High sensitivity
Kit Contents |
Amount |
Storage |
Reaction wells |
12x8 (96 total) |
RT(25˚C+/- 2.5) (Dry and Dark) |
Test strips |
48x2 (96 total) |
|
Positive control |
1 vial |
|
Negative control |
1 vial |
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink or light red color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the antibiotic binding proteins on the gold particles to engage with any antibiotic present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to their respective test line, forming a signal (red line) at that position. Each test strip is imprinted with three test lines and one Control line (C-line)at the top. The top test line specifically detects for beta-lactams, the middle test line detects for tetracyclines, and the bottom test line detects for sulfonamide antibiotics. If the binding protein on the gold particle has engaged with either beta-lactams,tetracyclines, or sulfonamide antibiotic residues present in the milk sample, the gold particle will flow past the test line corresponding to that antibiotic and reach the C-line.
Negative: Test line and the C-line both appear ,indicates the milk sample is negative .
Positive: Test line which corresponding position is no colored and the C-line appear, indicates the milk sample is positive for the corresponding drug .
The test is invalid if no colored line appears in the C-line area even if a colored line appears in the test line area. Re-test milk sample if broken lines are observed. Colored lines which appear after 20 minutes are not diagnostic and should be ignored. If the sample does not flow up the dipstick, the milk sample may be clotted, invalidating the test result. To avoid milk sample clotting, always use fresh milk.