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| Place of Origin | USA |
| Brand Name | REAGEN |
| Certification | FAPAS |
| Model Number | RNM98004 |
REAGEN™DON ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals,feed.
1. High recovery (75% - 105%), and cost-effective extraction methods .
2. High reproducibility.
3. A quick ELISA assay (less than0.5 hour regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The DON-BSA has been coated in the plate wells. During the analysis, sample and HRP-conjugated antibody 2# are added along with the antibody 1#. If the DON residue is present in the sample, it will compete for the DON antibody 1#, thereby preventing the DON-BSA attached to the well from binding to the antibody 1#. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the DON residue concentration in the sample.
REAGEN™DON ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| DON -BSA-Coated Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8°C |
|
DON Standards: Negative control (white cap tube) 2 ng/mL (yellow cap tube) 6ng/mL (orange cap tube) 18 ng/mL (pink cap tube) 54 ng/mL (purple cap tube) 162 ng/mL (blue cap tube) 1000 ng/mL (spiking, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
| Antibody 1# | 6 mL | 2-8°C |
| HRP Conjugated Antibody 2# | 6 mL | |
| 10X Sample Dilution Buffer | 10 mL | |
| 20X Wash Solution | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 m |
| Sample Type | Detection Limit (ng/g or ppb) |
| Cereals/Feed | 80 |
| Analytes | Cross-Reactivity (%) |
| DON | 100 |
| 3-AC-DON | >100 |
| 15-AC-DON | 4.2 |
| Triacetyl-DON | 0.5 |
| T-2 toxin | <0.1 |
| T-2 triol | <0.1 |
| Nivalenol | <0.1 |
The standards contain DON. Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25°C / 68 – 77°F) while in the packaging.
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