The premixed solution significantly improves the efficiency and accuracy of the experiment, and has the characteristics of ease of use, flexibility, accuracy, high efficiency and easy operation. The kit allows for seamless assembly of multiple DNA fragments without fragment length or end compatibility limitations, especially when assembling single-stranded oligonucleotides or DNA fragments of different sizes with different overlaps (15-80 bp).
1.The whole process takes less than 2 hours
2.Flexible sequence design (scar free cloning)
3.No PCR purification steps required
4.High conversion efficiency, suitable for gene fragments up to 20 kb
5.Easy to adapt to multiple DNA manipulations, including site-specific mutagenesis
6.Includes receptive cells